Methods used for the study of viruses
Multiplicity of Infection
Influenza virus growth in eggs
Influenza hemagglutination inhibition assay
Influenza microneutralization assay
Detecting viruses: the plaque assay
Measurement of viruses by end-point dilution assay
The western blot
Detection of antigens or antibodies by ELISA
Detecting viral proteins in infected cells or tissue by immunostaining
Introduction + Homology
Introduction to multiple sequence alignment
Viral bioinformatics: Multiple sequence alignment – Jalview
Viral bioinformatics: Multiple sequence alignment €“ Base-By-Base (BBB) editor
Viral bioinformatics: Sequence searcher
17 thoughts on “Virology Toolbox”
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This page is an excellent idea.
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This is a great idea and great help for starters at virological field!
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Great info sir..
Hello Dr., I need to know how did u calculate the equation 4 the plaque assayplz?
are there any videos about virus vs. bacteria
i would like to know how to detect the antiviral activity?
I do have a practical problem. I measured TCID50 value of my virus (1.6X10^4/ml) now like to use it as 1 TCID50 or 100 TCID50. My final volume of infection is 100 microliter. How much virus I need to add from the above stock. Does the number of cell(s) in the culture influence the calculation. Please discuss anybody who ever interested. Thanks.
Do you have recommendations or resources for protocols (books or online)? I am also looking for a resource to learn about the cell lines and strains commonly used in virology research.
great work!!! love this blog always 🙂
Let me present a pre-clinical animal model using
primates. I donâ€™t know if you have any exposure but it would lead to
further clinical trials which could be strategic in your region. Please
see the following message:
Do you have capabilities for. a pre-clinical study, to help
in an Ebola study using primates?
The design is very simple.
We were told that most trials have been using 4
primates. The primates are exposed to Ebola, then the test articles are
administered and survivability is noted.
In this case we want to test as a prophylactic. That
would mean administering the test article to the group, then exposing them to
Very frankly I am not certain on the controls. Two
controls, two with prophylactic?
We need to talk to the P.I.
Our test article is based on a formula we used a few years
back that was successful with HIV. We understand the Ebola virus and have
modified the formula through our in silico process. We are in the middle
of the in vitro assays which will be finished mid February and must prepare to
be ready for the NHP phase. We need a door opened at a level 4 laboratory
that handles Ebola. firstname.lastname@example.org