Virology Toolbox

Methods used for the study of viruses

Viral growth

Multiplicity of Infection
Influenza virus growth in eggs

Viral detection
Influenza hemagglutination inhibition assay
Influenza microneutralization assay
Detecting viruses: the plaque assay
Measurement of viruses by end-point dilution assay
The western blot
Detection of antigens or antibodies by ELISA
Detecting viral proteins in infected cells or tissue by immunostaining

Viral Bioinformatics
Introduction + Homology
Introduction to multiple sequence alignment
Viral bioinformatics: Multiple sequence alignment – Jalview
Viral bioinformatics: Multiple sequence alignment €“ Base-By-Base (BBB) editor
Viral bioinformatics: Sequence searcher

17 thoughts on “Virology Toolbox”

  1. Pingback: Virology toolbox – the western blot

  2. Pingback: Viral bioinformatics: Introduction + Homology

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  4. Pingback: Viral bioinformatics: Recombination

  5. Pingback: Viral Bioinformatics: Multiple sequence alignment – Jalview

  6. Pingback: Are all virus particles infectious?

  7. I do have a practical problem. I measured TCID50 value of my virus (1.6X10^4/ml) now like to use it as 1 TCID50 or 100 TCID50. My final volume of infection is 100 microliter. How much virus I need to add from the above stock. Does the number of cell(s) in the culture influence the calculation. Please discuss anybody who ever interested. Thanks.

  8. Do you have recommendations or resources for protocols (books or online)? I am also looking for a resource to learn about the cell lines and strains commonly used in virology research.

  9. Hi:

    Let me present a pre-clinical animal model using
    primates. I don’t know if you have any exposure but it would lead to
    further clinical trials which could be strategic in your region. Please
    see the following message:

    Do you have capabilities for. a pre-clinical study, to help
    in an Ebola study using primates?

    The design is very simple.

    We were told that most trials have been using 4
    primates. The primates are exposed to Ebola, then the test articles are
    administered and survivability is noted.

    In this case we want to test as a prophylactic. That
    would mean administering the test article to the group, then exposing them to

    Very frankly I am not certain on the controls. Two
    controls, two with prophylactic?

    We need to talk to the P.I.

    Our test article is based on a formula we used a few years
    back that was successful with HIV. We understand the Ebola virus and have
    modified the formula through our in silico process. We are in the middle
    of the in vitro assays which will be finished mid February and must prepare to
    be ready for the NHP phase. We need a door opened at a level 4 laboratory
    that handles Ebola.

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