recombination

TWiEVO 55: Coronavirus evolution from soup to nuts

Nels and Vincent continue their discussion of SARS-CoV-2 evolution, with a report that the coronavirus proofreading enzyme stimulates RNA recombination, and debunking the conclusion that a change in the viral spike glycoprotein is associated with increased human to human transmission. Click arrow to play Download TWiEVO 55 (45 MB .mp3, 74 min) Subscribe (free): iTunes, Google Podcasts, RSS, email …

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TWiV 512: Flexuous SUMO wrestlers

Anne Simon joins the TWiV team to talk about plant viruses, including plum pox virus that devastates nut and stone fruit trees, and a geminivirus protein that regulates viral DNA synthesis. <span data-mce-type=”bookmark” style=”display: inline-block; width: 0px; overflow: hidden; line-height: 0;” class=”mce_SELRES_start”></span>&lt;span data-mce-type=”bookmark” style=”display: inline-block; width: 0px; overflow: hidden; line-height: 0;” class=”mce_SELRES_start”&gt;&lt;/span&gt;&amp;lt;span data-mce-type=”bookmark” style=”display: inline-block; …

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The purity of plaques

The plaque assay – my favorite assay in the world – is a time-honored procedure to determine the number of viruses in a sample, and to establish clonal virus stocks. The  linear relationship between the number of infectious particles and the plaque count (illustrated; image credit) shows that one infectious particle is sufficient to initiate infection. Despite the …

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Genome recombination across viral families

 A novel coronavirus isolated from bats in China is unusual because the genome contains a gene from a virus in a completely different family, the Reoviridae (link to paper). The finding suggests that recombination occurred between a (+) strand RNA virus and a virus with a segmented, double-stranded RNA genome. The unusual recombinant virus was identified …

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