A more rapid method than Western blot analysis to detect a specific protein in a cell, tissue, organ, or body fluid is enzyme-linked immunosorbent assay, or ELISA. This method, which does not require fractionation of the sample by gel electrophoresisis, is based on the property of proteins to readily bind ...

Hosts: Vincent Racaniello and Dickson Despommier On episode 13 of the podcast This Week in Parasitism, Vincent and Dickson continue their discussion of the obligate intracellular protozoan Toxoplasma gondii with a consideration of the clinical consequences of infection and pathogenesis. [powerpress url="http://traffic.libsyn.com/twip/TWiP013.mp3"] Click the arrow above to play, or right-click to ...

Global fish farming may be the solution to the impending collapse of the commercial fishing industry, but penned fish are susceptible to infectious diseases. Infection with salmon infectious anemia virus, an orthomyxovirus, lead Wal-Mart to stop buying farmed salmon from Chile, the world's second largest producer of the fish. As a ...

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Eric F. Donaldson On episode #90 of the podcast This Week in Virology, Vincent, Alan, Rich and Eric discuss identification of viruses in Northeastern American bats, vaccinia virus infection after sexual contact with a military vaccinee, and identification of a new flavivirus from ...

Readers of virology blog often request explanations of specific experimental techniques. Methods such as complement fixation, deep sequencing, ELISA, PCR and many others are frequently mentioned on this blog without discussion. To do so would interrupt the scientific discourse and make for lengthly posts. To remedy this shortcoming, I have ...