viral

Measurement of viruses by end-point dilution assay

The plaque assay is a terrific method for determining virus titers, but it doesn’t work for all viruses. Fortunately there are several alternative methods available, including the end-point dilution assay. The end-point dilution assay was used to measure virus titer before the development of the plaque assay, and is still used for viruses that do …

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TWiV 40: Tamiflu in the water

Hosts: Vincent Racaniello, Dick Despommier, and Alan Dove On episode #40 of the podcast “This Week in Virology”, Vincent, Dick, and Alan consider Reston ebolavirus in swine, historical perspective of H1N1 influenza virus emergence and circulation, Tamiflu-resistant H1N1, Tamiflu in Japanese river waters, transmission of H1N1 virus in ferrets, and pneumonia and respiratory failure from H1N1 …

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Detecting viruses: the plaque assay

One of the most important procedures in virology is measuring the virus titer – the concentration of viruses in a sample. A widely used approach for determining the quantity of infectious virus is the plaque assay. This technique was first developed to calculate the titers of bacteriophage stocks. Renato Dulbecco modified this procedure in 1952 …

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TWiV 39: Virus structure

Hosts: Vincent Racaniello and Dick Despommier On episode #39 of the podcast “This Week in Virology”, Vincent and Dick discuss virus structure and answer listener questions. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV039.mp3″] Click the arrow above to play, or right-click to download TWiV #39 (37 MB .mp3, 53 minutes) Subscribe to TWiV in iTunes, by the RSS feed, or by email Links …

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