Kartik Chandran and Rohit Jangra join the TWiV team to present their identification of protocadherin-1 as a cell receptor for New World hantaviruses. Click arrow to play Download TWiV 549 (62 MB .mp3, 103 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Show notes at microbe.tv/twiv
Of all the potential uses for genome editing via CRISPR/Cas9, perhaps the most exciting involves introducing therapeutic changes into human pluripotent stem cells (hPSCs) which can then be differentiated into many different cell types. A serious obstacle to such engineering is the finding that the tumor suppressor p53 inhibits CRISPR/Cas9 editing in hPSCs.
Erin Garcia joins the TWiVirions to discuss a computer exploit encoded in DNA, creation of pigs free of endogenous retroviruses, and mutations in the gene encoding an innate sensor of RNA in children with severe viral respiratory disease. Click arrow to play Download TWiV 455 (64 MB .mp3, 105 min) Subscribe (free): iTunes, RSS, email Become a patron of TWiV! Show notes …
There aren’t enough human organs to meet the needs for transplantation, so we have turned to pigs. Unfortunately pig cells contain porcine endogenous retroviruses, PERVS, which could infect the transplant recipient, leading to tumor formation. But why worry? Just use CRISPR to purge the PERVs. The genomes of many species on Earth are littered with endogenous …
The TWiVsters describe a new animal model for MERS coronavirus-induced acute respiratory distress syndrome, produced by CRISPR/Cas9 editing of the mouse gene encoding an ortholog of the virus receptor. You can find TWiV #418 at microbe.tv/twiv, or listen below. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV418.mp3″] Click arrow to play Download TWiV 418 (63 MB .mp3, 104 min) Subscribe (free): iTunes, RSS, email Become a patron of TWiV!
The TWiVeroos examine a reverse spillover of Newcastle disease virus vaccines into wild birds, and identification of a protein cell receptor for murine noroviruses. You can find TWiV #411 at microbe.tv/twiv, or listen below. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV411.mp3″] Click arrow to play Download TWiV 411 (59 MB .mp3, 95 min) Subscribe (free): iTunes, RSS, email Become a patron of TWiV!