Virus Watch: Counting Viruses
In this episode of Virus Watch, I show how to do my favorite assay in all of virology – the plaque assay.
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Detecting viruses: the plaque assay
One of the most important procedures in virology is measuring the virus titer – the concentration of viruses in a sample. A widely used approach for determining the quantity of infectious virus is the plaque assay. This technique was first developed to calculate the titers of bacteriophage stocks. Renato Dulbecco modified this procedure in 1952 …