So far in my experiments to understand inhibition of rhinovirus replication by ZnCl2 I’ve found that at a concentration of 0.1 mM, viral plaque formation is inhibited but not sufficiently to be able to select resistant mutants. Attempts to use higher concentrations of the metal have consistently failed. When I initially I tried higher concentrations …
Hosts: Vincent Racaniello and Dickson Despommier Vincent and Dick continue Virology 101 with a discussion of how RNA viruses produce mRNA and replicate their genomes. [powerpress url=”http://traffic.libsyn.com/twiv/TWiV060.mp3″] Click the arrow above to play, or right-click to download TWiV #60 (51 MB .mp3, 71 minutes) Subscribe to TWiV in iTunes, by the RSS feed, or by …
Each year I teach basic virology to medical, dental, and nursing students here at Columbia University Medical Center. You can find all the lecture videos, slides, and readings here at virology blog: virology.ws/course.
X-linked adrenoleukodystrophy (ALD) is a rare neurologic disease caused by a defect in a gene required for normal ABCD1 transporter function. The lack of this function leads to progressive demyelination, severe neurologic disease and death in males, often in childhood. ALD disease progression can be controlled by allogeneic hematopoietic cell transplantation (HCT) in those patients …
The Norwegian Institute of Public Health recently identified a mutation in 2009 H1N1 influenza virus isolated from two patients who died and one with severe disease. It has been suggested that this mutation, which causes a change from the amino acid aspartic acid to glycine at position 225 of the viral HA protein (D225G), could …
The D225G change in 2009 H1N1 influenza virus is not a concern Read More »
Recently I began experiments to understand how zinc inhibits rhinovirus replication, and I promised to document my findings on the pages of this blog. Here are the results of the second plaque assay. In the last experiment I confirmed the finding that 0.1 mM ZnCl2 inhibits plaque formation by rhinovirus type 1A. Based on the …
